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dnaA [2017-05-10 11:12:17]
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dnaA [2017-05-10 11:12:17]

AAA ATPase, replication initiation protein
locus
BSU00010
pI
6.03
mw
50.70 kDa
protein length
446 aa Sequence Blast
gene length
1338 bp Sequence Blast
function
DNA replication
product
replication initiation protein
essential
yes
synonyms
dnaH,dnaJ,dnaK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    410 → 1,750

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • binds multiple regions in the oriC region, required for recruitment of proteins needed to load the replicative helicase [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC]

    • Protein family

  • dnaA family (according to Swiss-Prot)

    • [SW|Domains]

  • AAA + domain

    • Effectors of protein activity

  • [protein|03595BF6DB8AE79604A720A5BD85C0DB781EE306|SirA] displaces [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] from the replication origin [Pubmed|19682252]
  • [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|YabA] inhibits co-operative binding of DnaA to the ''oriC'' DNA [Pubmed|21895792]
  • DnaA helix formation (and thus replication initiation) is inhibited by the interaction of either [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|ParA], [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|YabA] or [protein|83E05071DEC874248D3E96B8F3A093C65939B314|DnaN] with the AAA domain of DnaA [Pubmed|23909787,22286949]
  • interaction with [protein|C9FEE07601AD28B2651EB881A1D9B34D5082237C|DnaD] inhibits the ability of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] to cooperatively bind to DNA [Pubmed|22821970]

    • Structure

  • [PDB|2Z4R] (from ''Thermotoga maritima '') [Pubmed|18216012]

    • [SW|Localization]

  • throughout the cytoplasm [Pubmed|10844689]

    • Expression and Regulation

    Operons

    genes
    [gene|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-[gene|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]
    description
    [Pubmed|2987848]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • negatively controlled by [protein|search|DnaA] [Pubmed|2168872] and [SW|Spo0A] [Pubmed|14651647]

    • additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''dnaA'' [Pubmed|27120414]
    • view in new tab

    Labs working on this gene/protein

  • [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
  • [SW|Alan Grossman], MIT, Cambridge, MA, USA
  • [SW|Heath Murray], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]

    • References

    Reviews

  • 20157337,21035377,21639790,22575476,22797751,26706151,28075389

    • The [SW|DnaA regulon]

  • 16120674

    • Original publications

  • 20511500,21097613,21239581,21895792,22286949,22821970,23909787,22396664,21911367,25041308,25340815,26020636,18854156,19011033,11222620,14651647,17140409,10844689,11222620,12060778,16461910,2987848,11207367,19737352,19081080,17932079,19968790,19682252,18216012,2168872,26615189,27120414,27489185,27902860